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Nis Elements Viewer, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 39694 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nis-elements+viewer/NIS-Elements/pmc13039486-341-11-13
Average 99 stars, based on 39694 article reviews
Nis Elements Viewer, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 39694 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nis-elements+viewer/NIS-Elements/pmc13039486-341-11-13
Average 99 stars, based on 39694 article reviews
nis elements viewer - by Bioz Stars,
2026-09
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Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: RNAscope:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: Immunohistochemistry:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: Staining:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: CRISPR:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: Knock-Out:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: Recombinant:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: Cell Counting:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: Reverse Transcription:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: Whole Genome Amplification:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: Virus:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: ATP Assay:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: Saline:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: Cloning:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: Plasmid Preparation:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: Gel Extraction:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: Mutagenesis:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: Enzyme-linked Immunosorbent Assay:Article Title: FOXC1 and FOXC2 maintain mitral valve endothelial cell junctions, extracellular matrix, and lymphatic vessels to prevent myxomatous degeneration Article Snippet: WM and IHC staining images were acquired using a Article Title: Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy. Article Snippet: Zhou et al., STAR Protocols 6, Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects.. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photonmicroscope.. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. Article Title: FOXC1 and FOXC2 Ablation Causes Abnormal Valvular Endothelial Cell Junctions and Lymphatic Vessel Formation in Myxomatous Mitral Valve Degeneration Article Snippet: WM, RNAscope, and immunohistochemistry staining images were acquired using a Article Title: Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: Article Title: In vivo CRISPR screening directly targeting testicular cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms 10x Genomics Cell Ranger 6.0.0 Zheng et al.78 https://github.com/10XGenomics/cellranger ApE Davis et al.79 https://jorgensen.biology.utah.edu/wayned/ape/ CRISP-ID v1.1 Dehairs et al.80 http://crispid.gbiomed.kuleuven.be Customized python script for statistical analysis This paper https://github.com/SuzukiLab-icems/ utils_for_Cell_Genomics_2024 Cutadapt v4.1 Martin et al.81 https://github.com/marcelm/cutadapt DAVID Sherman et al.82 https://david.ncifcrf.gov docker-cellranger litd/docker-cellranger https://github.com/litd/docker-cellranger DSIR Vert et al.83 http://biodev.cea.fr/DSIR/DSIR.html FastQC v0.12.1 Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ FlowJo BD Life Sciences https://www.flowjo.com goatools v1.2.4 Klopfenstein et al.84 https://github.com/tanghaibao/goatools guide-caller v1.0.0 This paper https://github.com/SuzukiLab-icems/guide-caller HISAT2 v2.2.1 Perțea, M et al.85 http://daehwankimlab.github.io/hisat2/ Hub-Explorer v1.0.0 This Article Title: Biomanufacturing of glioblastoma organoids exhibiting hierarchical and spatially organized tumor microenvironment via transdifferentiation. Article Snippet: Funding information National Science Foundation, Grant/Award Number: 2000053; U.S. Department of Education, Grant/Award Number: P200A210069 Abstract Glioblastoma (GBM) is the most aggressive type of brain tumor that originates from glioblastoma stem cells (GSCs).. In the brain, GSCs are supported by a tumor microenvironment (TME) residing in the perivascular niche and the hypoxic niche.. The GBM TME is highly heterogenous and exhibits complex cell‐to‐cell interactions. Article Title: Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice Article Snippet: |